We now have numerous autostereoscopic displays, and it is mandatory to characterize them because it will allow to optimize their performances and to make efficient comparison between them. Therefore we need standards so we have to be able to quantify the quality of the viewer's perception. The purpose of the present paper is twofold; we first present a new instrument of characterization of the 3D perception on a given autostereoscopic displays; then we propose a new way to realize an experimental protocol allowing to get a full characterization. This instrument will allow us to compare efficiently the different autostereoscopic displays but it will also validate practically the adequacy between the shooting and rendering geometries. In this aim, we are going to match a perceived scene with the virtual scene. It is hardly possible to determinate the scene perceived by a viewer placed in front of an autostereoscopic display. Indeed if it may be executable on the pop-out, it is impossible on the depth effect because the depth of the virtual scene is set behind the screen. Therefore, we will have to use an optical illusion based on the deflection of light by a mirror to know the position which the viewer perceives some points of the virtual scene on an autostereoscopic display.